Product Description
![ZymoBIOMICS DNA Micro Kit <br> without Lysis Matrix, 50 Preps<br> Zymo D4305 ZymoBIOMICS DNA Micro Kit <br> without Lysis Matrix, 50 Preps<br> Zymo D4305](https://s.turbifycdn.com/aah/yhst-12533177228474/zymobiomics-dna-micro-kit-without-lysis-matrix-50-preps-zymo-d4305-11.gif)
ZymoBIOMICS DNA Micro Kit
without Lysis Matrix, 50 Preps
Zymo D4305
Storage Temperature: Room Temperature
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Applicable For: All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume: ≥ 10 µl ZymoBIOMICS DNase/RNase Free Water
Equipment: Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended).
Processing Volume: Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells)
Purity: High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8
Sample Source: Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.
Sample Storage: Eluted DNA should be stored at ≤ -20°C.
Size Range: Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Type: Total DNA
Yield: Up to 5 µg total DNA can be eluted into ≥ 10 µl