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ZymoBIOMICS DNA Microprep Kit
with Lysis Tubes, 50 preps
Zymo D4301
with Lysis Tubes, 50 preps
Zymo D4301
Product Description
ZymoBIOMICS DNA Microprep Kit
with Lysis Tubes, 50 preps
Zymo D4301
Storage Temperature: Room Temperature
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard. Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application. Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Applicable For: All sensitive downstream applications such as qPCR and Next-Generation Sequencing. Elution Volume: ≥ 10 µl ZymoBIOMICS DNase/RNase Free Water Equipment: Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). Processing Volume: Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) Purity: High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 Sample Source: Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. Sample Storage: Eluted DNA should be stored at ≤ -20°C. Size Range: Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. Type: Total DNA Yield: Up to 5 µg total DNA can be eluted into ≥ 10 µl
with Lysis Tubes, 50 preps
Zymo D4301
Storage Temperature: Room Temperature
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of tough microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard. Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application. Certified Low Bioburden: Boost your detection limit for low abundance microbes.
Applicable For: All sensitive downstream applications such as qPCR and Next-Generation Sequencing. Elution Volume: ≥ 10 µl ZymoBIOMICS DNase/RNase Free Water Equipment: Microcentrifuge, vortex/Disruptor Genie, high speed cell disruptor (recommended). Processing Volume: Fecal ≤200 mg, Soil ≤ 250 mg, Cells ≤ 100 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) Purity: High quality, inhibitor-free DNA. Typical A260/A280 ≥1.8 Sample Source: Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. Sample Storage: Eluted DNA should be stored at ≤ -20°C. Size Range: Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. Type: Total DNA Yield: Up to 5 µg total DNA can be eluted into ≥ 10 µl
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