TRIS Hydrochloride
[Tris(hydroxymethyl) aminomethane HCl],
250 Grams
RP- T60050-250.0

TRIS Hydrochloride
[Tris(hydroxymethyl) aminomethane HCl],
250 Grams
RP- T60050-250.0

Research or further manufacturing use only, not for food or drug use.

Common Name: Tris(hydroxymethyl) aminomethane hydrochloride

CAS Number: 1185-53-1

Molecular Weight: 157.6

Chemical Formula: C4H11NO3 • HCl

Solubility: Water

Storage Temperature: Room Temperature

TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.

It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.

TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.

One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.

Appearance: White crystalline Assay: 99.0 - 101.0 % pH (1% aqueous solution): 4.2 - 5.0 Solubility (1M Solution in Water ): Clear, colorless solution UV Absorbance @260nm, 1.0 M in H2O: ≤ 0.06 UV Absorbance @280nm, 1.0 M in H2O: ≤ 0.05 Identification: Conforms Heavy Metals: ≤ 5 ppm Grade: USP BSE/TSE Free: BSE/TSE Free