Tris Base Ultra Pure
[Tris (Hydroxymethyl) Aminomethane],
250 Grams
RP-T60040-250.0

Tris Base Ultra Pure
[Tris (Hydroxymethyl) Aminomethane],
250 Grams
RP-T60040-250.0

Research or further manufacturing use only, not for food or drug use.

Common Name: Tris (Hydroxymethyl) Aminomethane

CAS Number: 77-86-1

Molecular Weight: 121.1

Chemical Formula: C4H11NO3

Solubility: Water

Storage Temperature: Room Temperature

Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.

It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.

The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.

It is also utilized in the preparation of electrophoresis buffers and PCR reactions.

Moisture: <0.2% Color: <20 APHA (colorless) Heavy Metals (as Pb): <0.0001% Insoluble Matter: <0.005% Heavy Metals: 5 ppm pKa (20°C): 8.1 ± 0.1

Appearance: White Crystalline Powder Purity (Dried Basis By GC): ≥ 99.9 % Related Substances By GC: ≤ 0.1 % Water: ≤ 0.2 % Loss on Drying @ 105 oC, 3hrs: ≤ 1.0 % Melting Point: 170 - 172 oC APHA Color, 20% Aqueous solution: ≤ 20 Insoluble Matter: ≤ 0.005 % Heavy Metals (as Pb): ≤ 5 ppm Residue on Ignition: ≤ 0.5 % Iron (Fe): ≤ 5 ppm UV Absorbance @260nm, 10% Aqueous solution: ≤ 0.03 UV Absorbance @280nm, 10% Aqueous solution: ≤ 0.02 UV Absorbance @290nm, 40% solution in H2O: ≤ 0.20 UV Absorbance @430nm, 10% Aqueous solution: ≤ 0.004 Identification A: Pass Identification B: Pass Identification C: Pass pH (5% in water): 10.0 - 11.5 Arsenic (As): ≤ 1 ppm Chloride: ≤ 100 ppm Copper (Cu): ≤ 1 ppm DNase, RNase, protease: None Detected Lead: ≤ 1 ppm Magnesium (Mg): ≤ 1 ppm pH (1M in H2O): 10.5 - 11.5 Residual Solvents: Conforms to USP/EP Residue on Ignition (USP): ≤ 0.1 % Solubility (2M In H2O): Pass BSE/TSE Free: BSE/TSE Free