Quick-DNA Midiprep Plus Kit,
25 Preps
Zymo D4075

Quick-DNA Midiprep Plus Kit,
25 Preps
Zymo D4075

Storage Temperature: Room Temperature

The Quick-DNA Miniprep Plus Kit is the easiest method for high yield total DNA extraction (e.g., genomic, plasmid, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo-Spin Column technologies allow for ultra-pure and concentrated genomic DNA ˃ 50 kb to be eluted in as little as 35 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free, bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.

Extract high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, or solid tissues. Zymo-Spin Technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.

Elution Volume: As little as 200 µl DNA Elution Buffer or water. Size Range: Capable of recovering genomic and mitochondrial DNA sized fragments ˃ 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered. Equipment: Water bath or heat block (55˚C), centrifuge or vacuum source and manifold, microcentrifuge, vortex, conical tubes (15 – 50 ml), and microcentrifuge tubes. Purity: High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Generation Sequencing, bisulfite conversion, etc. (A260/A230 ≥ 2.0). Yield: The DNA binding capacity of the column is 125 μg. Typically, mammalian tissues yield: 1-3 μg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 μg DNA per mg liver and kidney. Human whole blood will yield 3-7 μg DNA per 100 μl blood sampled. Workflow: Utilizes a Proteinase K Digestion and Zymo-Spin Technology for effective recovery of DNA.