Product Description
Quick DNA Plus Microprep Kit,
50 Preps
Zymo D4074
Storage Temperature: Room Temperature
The Quick-DNA Microprep Plus Kit is the easiest method for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA > 50 kb to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
Extract high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, or solid tissues.
Zymo-Spin Technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
Equipment: Water bath or heat block (55˚C), microcentrifuge, and vortex.
DNA Purity: High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Generation Sequencing, bisulfite conversion, etc. (A260/A230 ≥ 2.0).
Elution Volume: DNA can be eluted into as little as 10 µl DNA Elution Buffer or water.
DNA Yield: The DNA binding capacity of each column is 5 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.
DNA Size: Capable of recovering genomic and mitochondrial DNA sized fragments ˃ 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.
DNA Applications: DNA isolated using the Quick-DNA Microprep Plus Kit can be used for life-science research, genotyping, livestock breeding, veterinary research, and routine applied testing among a variety of other applications.
DNA Types: The Quick-DNA Microprep Plus Kit will isolate total DNA including genomic, mitochondrial, plasmid, viral, parasitic, etc. from biological fluids, cells, or tissues. Not recommended for small cell-free DNA isolation from urine, serum, and plasma.
Workflow Overview: Utilizes a Proteinase K Digestion and Zymo-Spin Technology for effective recovery of DNA.