Product Description
DNA Clean & Concentrator-5
with Zymo-Spin IC Columns,
Capped, 200 Preps
Zymo D4014
Storage Temperature: Room Temperature
The DNA Clean & Concentrator-5 is a PCR purification kit that provides purification of up to 5 µg DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. This product facilitate the removal of DNA polymerases, modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, and restriction endonucleases, as well as free dNTPs and their analogs including radiolabeled and fluorescent derivatives. Eluted DNA is suitable for PCR, arrays, ligation, sequencing, etc.
Clean and concentrate up to 5 µg DNA with ≥ 6 µl elution in as little as 2 minutes with 0 µl wash residue carryover.
Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer.
Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Elution Volume: ≥ 6μl
Processing Time: 2 Minutes
Preps: 200
Format: Spin Column
DNA Recovery: Typically, ≤5 µg total DNA can be eluted with ≥6 µl water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%
Equipment: Microcentrifuge
DNA Size Limits: 50 bp to 23 kb
Sample Sources: DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc
DNA Purity: Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions
Product Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS