DNA Clean & Concentrator-500,
10 Preps
Zymo D4031

DNA Clean & Concentrator-500,
10 Preps
Zymo D4031

Storage Temperature: Room Temperature

The DNA Clean & Concentrator-500 (DCC-500) is our highest capacity DNA Clean & Concentrator product. It is designed for the rapid, large format purification and concentration of up to 500 µg of high quality DNA from samples such as large-scale restriction endonuclease digestions and crude DNA preparations. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA transfection, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. The entire DNA purification/concentration procedure typically takes less than 20 minutes.

Simple, rapid recovery of ultra-pure DNA from large-scale sample sources. Unique column construction allows sample washing to be performed using a centrifuge or vacuum source. Column design allows DNA to be eluted at high concentrations into minimal volumes. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.

Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS Elution Volume: ≥ 2 ml Equipment: Microcentrifuge and centrifuge or vacuum source. Purity: Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. Sample Source: DNA from large-scale sample sources including restriction endonuclease digestions and "crude" DNA preparations from cell-free lysates. Size Range: 50 bp to 23 kb Yield: ≤ 500 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.