ZymoBIOMICS Microbial Community Standard II
(Log Distribution), Isolated DNA, 220 ng
Zymo D6311

ZymoBIOMICS Microbial Community Standard II
(Log Distribution), Isolated DNA, 220 ng
Zymo D6311

Storage Temperature: -20°C

ZymoBIOMICS Microbial Community Standard II (Log Distribution) is a mock microbial community consisting of eight bacterial and two fungal strains. This microbial standard can be used to assess the performance of microbiomics workflows and can also be used as a positive control for routine sequencing. Cells of the ten microbes were mixed in a log-distributed abundance, which allows the user to easily assess the detection limit of their microbiomics workflow. 75 μl of the standard contains about ~100 cells of the Staphylococcus aureus, the organism of lowest abundance. If needed, the standard can be spiked into a sample matrix (e.g. soil and blood) to mimic real samples of interest.

Theoretical Composition Based on Genomic DNA: Listeria monocytogenes - 89.1%, Pseudomonas aeruginosa - 8.9%, Bacillus subtilis - 0.89%, Saccharomyces cerevisiae - 0.89%, Escherichia coli - 0.089%, Salmonella enterica - 0.089%, Lactobacillus fermentum - 0.0089%, Enterococcus faecalis - 0.00089%, Cryptococcus neoformans - 0.00089%, and Staphylococcus aureus - 0.000089%

Log abundance distribution: assess detection limit of as low as DNA of three microbes Accurate composition: cross-validated with multiple types of measurements Microbiomics QC: ideal for quality control of microbiome measurements

Purity: Contains < 0.01% foreign microbial DNA. Yield: Not applicable. Sample Source: Genomic DNA of eight bacteria and two yeasts. Processing Volume: null Applicable For: NGS, microbiomics, metagenomics.