Product Description
ZymoBIOMICS MagBead DNA/RNA,
96 Preps
Zymo R2135
Storage Temperature: Room Temperature
ZymoBIOMICS MagBead DNA/RNA is a high-throughput microbiome DNA and RNA kit that provides magnetic bead-based DNA and RNA extraction for microbial DNA & RNA from any sample type. Efficiently isolate DNA and RNA from feces, soil, plant, water and biofilms that contain microbes such as gram-positive/negative bacteria, fungi, protozoans and viruses. In the ZymoBIOMICS workflow, DNA/RNA Shield is used to preserve nucleic acids from any sample at ambient temperatures and is coupled with an innovative lysis system (i.e., ZR BashingBead Lysis Tubes or Rack) for unbiased nucleic acid extraction. DNA & RNA is ready for any downstream microbiome or metagenome analyses.
Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.
Ultra-Pure: High quality DNA/RNA (including small/micro RNAs) are inhibitor-free and ready for qPCR and microbiome measurements using Next-Generation Sequencing
High Sensitivity: Increased detection limit of very low abundance organisms. Certified low bioburden.
Purity: High quality DNA and RNA (A260/A280 > 1.8, A260/A230 >1.8) are recovered.
Recovery: The DNA/RNA binding capacity is ~20 µg (per 30 µl MagBinding Beads).
Required Equipment: Centrifuge fitted with a 96 well microplate carrier, 96 well magnetic stand, 96 well plate heat block, liquid handler or other robotic sample processer (user supplied).
Sample Preservation: DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.
Sample type: Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50 mg of mammalian feces, ≤ 50 mg soil, and 5-20 mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs.
Size Limits: Capable of recovering DNA and total RNA ≥ 17 nucleotides.
Storage: DNA and RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤-70°C. The addition of RNase inhibitors is highly recommended for prolonged storage.