ZymoBIOMICS 96 MagBead DNA Kit
(No Lysis Matrix), 2 x 96 preps
Zymo D4306

ZymoBIOMICS 96 MagBead DNA Kit
(No Lysis Matrix), 2 x 96 preps
Zymo D4306

Storage Temperature: Room Temperature

The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.

High-throughput purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluids. The ZymoBIOMICS innovative lysis system enables efficient and unbiased lysis of microbes including Gram-positive/negative bacteria, fungi, protozoans, algae, etc. The automation friendly workflow enables nearly any sample to be processed in as little as 90 minutes for 96 preps.

Applicable For: All sensitive downstream applications such as qPCR and Next-Generation Sequencing. Elution Volume: ≥ 37.5 µl DNA Elution Buffer or DNase free water Equipment: Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block Processing Volume: Fecal ≤100 mg, Soil ≤ 200 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) Purity: High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing Sample Source: Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. Sample Storage: Eluted DNA should be stored at ≤ -20°C. Size Range: Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield. Type: Total DNA Yield: Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum)