XJb(DE3) Autolysis,
10 x 100 ul Mix & Go competent cells
and 1 ml 500X L-Arabinose
Zymo T3051

XJb(DE3) Autolysis, <br> 10 x 100 ul Mix & Go competent cells <br>and 1 ml 500X L-Arabinose <br> Zymo T3051
XJb(DE3) Autolysis,
10 x 100 ul Mix & Go competent cells
and 1 ml 500X L-Arabinose
Zymo T3051
Item# Zymo-T3051
Regular price: $285.00
Sale price: $269.99
Quantity&Size: 

Product Description

XJb(DE3) Autolysis, <br> 10 x 100 ul Mix & Go competent cells <br>and 1 ml 500X L-Arabinose <br> Zymo T3051
XJb(DE3) Autolysis,
10 x 100 ul Mix & Go competent cells
and 1 ml 500X L-Arabinose
Zymo T3051

HIGHLIGHTS :

Fast: 80 - 90% of E.coli are lysed in only 10 minutes after harvesting. High Transformation Efficiencies: Achieve 108 - 109 transformants per µg of plasmid DNA. DE3 Lysogen: Encodes the T7 polymerase for expressing recombinant proteins under the control of the T7 promoter.

DESCRIPTION :

While there are many cell lysis methods available to scientists, unfortunately none of these methods combine all the ideal features for simple, efficient, economical, and gentle lysis of E. coli cells. The E. coli XJ autolysing strains from Zymo Research were engineered to address this problem. Mild expression of a chromosomally encoded bacteriophage λ R gene, encoding the λ lysozyme, also known as λ endolysin, is induced during growth. Cells are harvested intact while the peptidoglycan layer of the cell walls has been protected from digestion by the cytoplasmic membrane. The membrane is, however, amenable to disruption by a brief physico-chemical stress such as a freeze-thaw cycle after harvesting the cells. The XJ Autolysis™ method is highly efficient and takes only minutes (unlike traditional multiple freeze-thaw cycles). It can be applied to any number of samples without increasing processing time and labor (unlike sonication or French-press), is reliable and repeatable (unlike lysozyme treatment), and finally, is fully compatible with a wide range of buffers. Additionally, it does not require use of any potentially interfering components such as detergents, commonly found in various lytic buffers. They are also applicable for nucleic acid purification, and available with a DE3 lysogen encoding the T7 polymerase for expressing recombinant proteins driven by the T7 promoter.

Accessories

XJb(DE3) Autolysis, <br> 10 x 100 ul Mix & Go competent cells <br>and 1 ml 500X L-Arabinose <br> Zymo T3051
Regular price: $285.00
Sale price: $269.99
XJb(DE3) Autolysis,
10 x 100 ul Mix & Go competent cells
and 1 ml 500X L-Arabinose
Zymo T3051
Zymo-T3051
Quantity&Size: 
XJb(DE3) Autolysis, with 1 Glycerol Stock  <br>and 1 ml 500X L-Arabinose <br> Zymo T5051
Regular price: $150.00
Sale price: $133.99
XJb(DE3) Autolysis, with 1 Glycerol Stock
and 1 ml 500X L-Arabinose
Zymo T5051
Zymo-T5051
Size: 
XJb Autolysis, 10 x 100 ul <br> Mix & Go competent cells <br>and 1 ml 500X L-Arabinose <br> Zymo T3041
Regular price: $300.00
Sale price: $279.99
XJb Autolysis, 10 x 100 ul
Mix & Go competent cells
and 1 ml 500X L-Arabinose
Zymo T3041
Zymo-T3041
Quantity: 
XJb Autolysis, with 1 Glycerol Stock <br> and 1 ml 500X L-Arabinose <br> Zymo T5041
Regular price: $150.00
Sale price: $133.99
XJb Autolysis, with 1 Glycerol Stock
and 1 ml 500X L-Arabinose
Zymo T5041
Zymo-T5041
Size: 
XJa(DE3) Autolysis, <br>10 x 100 ul Mix & Go competent cells <br>and 1 ml 500X L-Arabinose <br> Zymo T3031
Regular price: $285.00
Sale price: $269.99
XJa(DE3) Autolysis,
10 x 100 ul Mix & Go competent cells
and 1 ml 500X L-Arabinose
Zymo T3031
Zymo-T3031
Quantity&Size: 
XJa Autolysis, <br>10 x 100 ul Mix & Go competent cells  <br>and 1 ml 500X L-Arabinose <br> Zymo T3021
Regular price: $285.00
Sale price: $269.99
XJa Autolysis,
10 x 100 ul Mix & Go competent cells
and 1 ml 500X L-Arabinose
Zymo T3021
Zymo-T3021
Quantity&Size: 
XJa(DE3) Autolysis, <br> with 1 Glycerol Stock and 1 ml 500X L-Arabinose <br> Zymo T5031
Regular price: $150.00
Sale price: $133.99
XJa(DE3) Autolysis,
with 1 Glycerol Stock and 1 ml 500X L-Arabinose
Zymo T5031
Zymo-T5031
Size: 
XJa Autolysis, with 1 Glycerol Stock<br> and 1 ml 500X L-Arabinose <br> Zymo T5021
Regular price: $150.00
Sale price: $139.99
XJa Autolysis, with 1 Glycerol Stock
and 1 ml 500X L-Arabinose
Zymo T5021
Zymo-T5021
Size: