Quick-16S Plus NGS Library Prep Kit
(V3-V4, UDI), w/Primer Set 3, Rxns 96
Zymo D6421-PS3

Quick-16S Plus NGS Library Prep Kit  <br>(V3-V4, UDI), w/Primer Set 3, Rxns 96 <br> Zymo D6421-PS3
Quick-16S Plus NGS Library Prep Kit
(V3-V4, UDI), w/Primer Set 3, Rxns 96
Zymo D6421-PS3
Item# Zymo-D6421-PS3
Regular price: $1,500.00
Sale price: $1,399.99
Size: 

Product Description

Quick-16S Plus NGS Library Prep Kit  <br>(V3-V4, UDI), w/Primer Set 3, Rxns 96 <br> Zymo D6421-PS3
Quick-16S Plus NGS Library Prep Kit
(V3-V4, UDI), w/Primer Set 3, Rxns 96
Zymo D6421-PS3

The Quick-16S Plus NGS Library Prep Kit (V3-V4, UDI) is the fastest and simplest NGS library prep targeting the V3-V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups. Additional library quantification analysis such as TapeStation analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples. 100% automation ready with only a single PCR step and without the need for normalization. Real-time PCR enables absolute microbial copy number quantification.

16S V3-V4 Primer Sequences (adapters not included): 341f (CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG, 17 bp) and 806r (GACTACNVGGGTMTCTAATCC, 24 bp). The forward primer 341f is a mixture of the two sequences listed. Amplicon Size: The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~606 bp. Barcode Sequences: Barcode Sequences: 10 bp barcodes, Available for download here. Index Primers: Unique dual index (barcodes) to label individual samples. Primer Coverage: bacteria (84.2%), archaea (75.8%), and eukaryota (0.0%). Required Equipment: Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates. Sample Input: Purified microbial DNA ≤100 ng, free of PCR inhibitors. Sequencing Platform: Illumina MiSeq without the need to add custom sequencing primers. Zymo Research recommends the MiSeq Reagent Kit v3 (600-cycle). For assistance with sample sheet setup, see Appendix F.

Accessories

Quick-16S Plus NGS Library Prep Kit <br> (V3-V4 UDI) with Primer Set 1  <br> 96 rxns <br> Zymo D6421-PS1
Regular price: $1,500.00
Sale price: $1,399.99
Quick-16S Plus NGS Library Prep Kit
(V3-V4 UDI) with Primer Set 1
96 rxns
Zymo D6421-PS1
Zymo-D6421-PS1
Size: 
Quick-16S Plus NGS Library Prep Kit  <br>(V3-V4, UDI), w/Primer Set 2, Rxns 96 <br> Zymo D6421-PS2
Regular price: $1,500.00
Sale price: $1,399.99
Quick-16S Plus NGS Library Prep Kit
(V3-V4, UDI), w/Primer Set 2, Rxns 96
Zymo D6421-PS2
Zymo-D6421-PS2
Size: 
Quick-16S Plus NGS Library Prep Kit  <br>(V3-V4, UDI), w/Primer Set 3, Rxns 96 <br> Zymo D6421-PS3
Regular price: $1,500.00
Sale price: $1,399.99
Quick-16S Plus NGS Library Prep Kit
(V3-V4, UDI), w/Primer Set 3, Rxns 96
Zymo D6421-PS3
Zymo-D6421-PS3
Size: 
Quick-16S Plus NGS Library Prep Kit <br>(V3-V4, UDI), w/Primer Set 4, Rxns 96 <br> Zymo D6421-PS4
Regular price: $1,500.00
Sale price: $1,399.99
Quick-16S Plus NGS Library Prep Kit
(V3-V4, UDI), w/Primer Set 4, Rxns 96
Zymo D6421-PS4
Zymo-D6421-PS4
Size: 
Quick-16S Plus NGS Library Prep Kit  <br>(V3-V4, UDI), w/Primer Set 5, Rxns 96 <br> Zymo D6421-PS5
Regular price: $1,500.00
Sale price: $1,399.99
Quick-16S Plus NGS Library Prep Kit
(V3-V4, UDI), w/Primer Set 5, Rxns 96
Zymo D6421-PS5
Zymo-D6421-PS5
Size: 
Quick-16S Plus NGS Library Prep Kit <br> (V3-V4, UDI), w/Primer Set 6, Rxns 96 <br> Zymo D6421-PS6
Regular price: $1,500.00
Sale price: $1,399.99
Quick-16S Plus NGS Library Prep Kit
(V3-V4, UDI), w/Primer Set 6, Rxns 96
Zymo D6421-PS6
Zymo-D6421-PS6
Size: 
Quick-16S Plus NGS Library Prep Kit <br> (V3-V4, UDI), w/Primer Set 7, Rxns 96 <br> Zymo D6421-PS7
Regular price: $1,500.00
Sale price: $1,399.99
Quick-16S Plus NGS Library Prep Kit
(V3-V4, UDI), w/Primer Set 7, Rxns 96
Zymo D6421-PS7
Zymo-D6421-PS7
Size: 
Quick-16S Plus NGS Library Prep Kit <br> (V3-V4, UDI), w/Primer Set 8, Rxns 96 <br> Zymo D6421-PS8
Regular price: $1,500.00
Sale price: $1,399.99
Quick-16S Plus NGS Library Prep Kit
(V3-V4, UDI), w/Primer Set 8, Rxns 96
Zymo D6421-PS8
Zymo-D6421-PS8
Size: