Quick-DNA Microprep Kit,
200 Preps
Zymo D3021

Quick-DNA Microprep Kit,
200 Preps
Zymo D3021

Storage Temperature: Room Temperature

The Quick-DNA Kits are ideal DNA isolation kits for easy, rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these kits. Zymo-Spin Column/Plate technology enables high-quality DNA purification in minutes. PCR inhibitors are effectively removed, and the eluted DNA is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.

Easy purification of high-quality DNA from whole blood, buffy coat, swabs, or cultured cells. Protocol excludes the use of Proteinase K and organic denaturants for biofluid and cell samples. Eluted, inhibitor-free DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.

Elution Volume: ≥10 µl Equipment Microcentrifuge, Vortex Purity: High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 Sample Source: Whole blood, plasma, or serum from humans, mice, rats, etc. Cells from culture, buccal cells, as well as a variety of biological liquids are effectively processed using this kit. Tissue already digested with Proteinase K or mechanically homogenized. Size Range: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered Workflow: Unique lysis buffer system omits the need for Proteinase K digestion for biological fluids and cell culture samples. Yield: Up to 5 µg total DNA is eluted into ≥10 µl (6 µl minimum) DNA Elution Buffer or water. Human whole blood yields 1.5-3.5 µg DNA per 50 µl blood sampled. Mammalian tissues already homogenized will yield 1-3 µg DNA per mg.