Product Description
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Quick-DNA Fecal/Soil Microbe MicroPrep Kit,
50 Preps
Zymo D6012
The Quick-DNA Fecal/Soil Microbe Microprep Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
Rapid method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
Ultra-high density BashingBeads are fracture resistant and chemically inert.
Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
Applicable: For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume: ≥ 20 µl
Equipment: Microcentrifuge, vortex, cell disruptor/pulverizer
Processing Volume: ≤50 mg feces, ≤ 250 mg soil, ≤20 mg fungal/bacterial cells (wet weight)
Purity: A260/A280 nm ≥1.8.
Sample Source: Feces or soil
Sample Storage: DNA stored at ≤ -20°C.
Size Range: Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.
Type: Total DNA
Yield: ≤ 5 µg total DNA