PureRec Duplex-Specific Nuclease (DSN)
1000 Units
Zymo E1020-1000
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Product Description
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PureRec Duplex-Specific Nuclease (DSN)
1000 Units
Zymo E1020-1000
HIGHLIGHTS :
Highest Specificity: Selectively digests dsDNA and DNA in DNA-RNA hybrids; virtually inactive towards ssDNA or RNA.
Premium Purity: RNase-free, protease-free, and animal-free.
Thermostable: Able to withstand high temperatures without denaturation or loss in activity.
DESCRIPTION :
PureRec Duplex-Specific Nuclease (DSN) is an endonuclease that selectively digests double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrids, exhibiting virtually no activity towards single-stranded DNA (ssDNA) or RNA.
It can discriminate between perfectly matched and non-matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA-RNA duplex.
This enzymatic function is reliant upon the presence of Mg2+ ions, with a recommended concentration of 5 mM for most applications.
PureRec DSN is overexpressed and extensively purified from a non-animal source and is free of non-specific nucleases or RNases.
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APPLICATION :
Remove gDNA and DNA in DNA:RNA hybrids for protein or RNA preparations.
Reduce abundant rRNA and globin transcripts to enhance next-generation sequencing (NGS) libraries.
Achieve precise single-nucleotide polymorphism (SNP) detection with minimal off-target effects.
Enhance signal amplification for accurate virus detection.
Improve detection of miRNA and biomarkers.
Accessories
![PureRec Duplex-Specific Nuclease (DSN)<br> 200 Units <br> Zymo E1020-200 PureRec Duplex-Specific Nuclease (DSN)<br> 200 Units <br> Zymo E1020-200](https://s.turbifycdn.com/aah/yhst-12533177228474/purerec-duplex-specific-nuclease-dsn-200-units-zymo-e1020-200-12.gif) Regular price: $185.00 Sale price: $174.99 | ![PureRec Duplex-Specific Nuclease (DSN) <br> 1000 Units <br> Zymo E1020-1000 PureRec Duplex-Specific Nuclease (DSN) <br> 1000 Units <br> Zymo E1020-1000](https://s.turbifycdn.com/aah/yhst-12533177228474/purerec-duplex-specific-nuclease-dsn-1000-units-zymo-e1020-1000-8.gif) Regular price: $650.00 Sale price: $599.99 |