Research or further manufacturing use only, not for food or drug use.
Common Name: Phosphate Buffered Saline 10X Solution
CAS Number: 7732-18-5; 7647-14-5; 7447-40-7; 7558-79-4; 7778-77-0
Solubility: Water
Storage Temperature: Room Temperature
PBS 10X Solution, is a widely used reagent in life science research. It is a buffered saline solution that contains phosphate ions, which help maintain a stable pH level in biological and biochemical experiments.
PBS 10X Solution is commonly used in various applications such as cell culture, immunohistochemistry, immunocytochemistry, and molecular biology techniques. The primary function of PBS 10X Solution is to provide a stable environment for biological samples by maintaining a physiological pH and ionic strength.
The phosphate buffer system present in PBS helps to resist changes in pH caused by the addition of acids or bases, making it an essential tool for experimental procedures where pH control is crucial. PBS 10X Solution is prepared as a concentrated solution, typically at 10 times the final desired concentration. This concentrated form allows researchers to easily dilute it to the required working concentration for their specific experiments. It is available commercially from various suppliers and comes in different formats, such as liquid or powder form.
The versatility and reliability of PBS 10X Solution have made it a standard reagent in many laboratory protocols. Its use in cell biology and molecular biology research ensures consistent and reproducible results. PBS 10X Solution is a common choice for washing and resuspending cells, tissues, or protein samples, as it provides the necessary osmolarity and pH stability required for these procedures.
Appearance: Clear and Colorless Solution
Composition: Sodium Chloride: 1.37M, Potassium Chloride: 0.027M, Phosphate Buffer: 0.12M
Method: Prepared in 18.2 megaohams-cm ± 1 water and filtered through 1 micron filter
pH (1X Solution): 7.2 - 7.6
pH (10X Solution): 6.8 - 7.2