HEPES, Free Acid
[N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)],
25 Kilograms
RP-H75030-25000.0

HEPES, Free Acid
[N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)],
25 Kilograms
RP-H75030-25000.0

Research or further manufacturing use only, not for food or drug use.

Common Name: [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)]

CAS Number: 7365-45-9

Molecular Weight: 238.3

Chemical Formula: C8H18N2O4S

Solubility: Water

Storage Temperature: Room Temperature

HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.

In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.

HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.

One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.



pka (25�C): 7.5 pH Range (Working): 6.8 - 8.2 Heavy Metals: <0.001% Max

Appearance: White Crystalline Powder Assay (Titration, dried basis): ≥ 99.5 % DNase, RNase, protease: Not Detected Endotoxin (0.2% Solution): ≤ 0.25 EU/ml Heavy Metals (as Pb): ≤ 0.001 % Identification (IR): Pass Iron (Fe): ≤ 0.0005 % Loss on Drying: ≤ 1.0 % pH (5% in water): 5.0 - 6.5 pKa @ 20�C: 7.35 - 7.70 Residue on Ignition: ≤ 0.1 % Solubility (1M Solution in Water ): Clear and Colorless Solution Sulfate: ≤ 0.05 % UV Absorbance (1M@260nm): ≤ 0.05 UV Absorbance (1M@280nm): ≤ 0.05 BSE/TSE Free: BSE/TSE Free