EZ DNA Methylation-Lightning Kit,
50 Reactions
Zymo D5030

EZ DNA Methylation-Lightning Kit,
50 Reactions
Zymo D5030

Storage Temperature: Room Temperature

The EZ DNA Methylation-Lightning Kit can rapidly bisulfite convert and purify DNA in less than 1.5 hours. A ready-to-use conversion reagent streamlines this process – simply add the reagent directly to the sample and incubate. Desulphonation and clean-up of the converted DNA is performed on a spin-column, allowing an elution volume as low as 10 µl. The kit achieves high conversion efficiency of unmodified cytosines into uracil, ensuring accurate downstream methylation analysis.

Fastest bisulfite conversion kit for complete bisulfite conversion of DNA for methylation analysis. Ready-to-use conversion reagent is added directly to DNA. High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.

Applications: Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc. Conversion: > 99.5% Elution Volume ≥ 10 µl Equipment: Thermocycler with heated lid and microcentrifuge. Input: 100 pg - 2 µg of DNA. Processing Time: 1.5 hours Recovery: > 80% Sample Source: Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.