Product Description
EZ-96 DNA Methylation-Lightning Kit,
Shallow-Well, 2 x 96 Rxns
Zymo D5032
Storage Temperature: Room Temperature
The EZ-96 DNA Methylation-Lightning Kit is a rapid, high-throughput (96-well) bisulfite conversion kit for DNA methylation analysis. The streamlined workflow features ready-to-use Lightning Conversion Reagent and a combined DNA denaturation and bisulfite conversion process. Desulphonation and clean-up of the converted DNA is performed using a unique 96-well spin-plate. With the Deep-Well Kit, samples can be eluted in as little as 15 µl. High yield, converted DNA is ideal for PCR, array, and Next-Generation sequencing, etc.
Fastest 96-well bisulfite conversion kit for complete, high-throughput (96-well) bisulfite conversion of DNA for methylation analysis.
Ready-to-use conversion reagent is added directly to DNA.
High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.
Applications: Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-Generation sequencing, PCR amplification, etc.
Conversion: > 99.5%
Elution Volume: Deep-well: ≥ 15 µl Shallow-well: ≥ 30 µl
Equipment: Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input: 100 pg - 2 µg of DNA.
Processing Time: 1.5 hours
Recovery: > 80%
Sample Source: Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.