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DNA Degradase Plus,
1000 Units
Zymo E2021
1000 Units
Zymo E2021
Product Description
DNA Degradase Plus,
1000 Units
Zymo E2021
DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol
Assay Condition: DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour Concentration: 5 U/µl Enzyme Inactivation: Heat inactivate enzyme at 70C for 20 minutes Storage: Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C. Unit Definition: 5 U of enzyme will digest 1 µg of genomic DNA in 25 µl reaction volume at 37C for ≥ 1 hr
1000 Units
Zymo E2021
DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol
Assay Condition: DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour Concentration: 5 U/µl Enzyme Inactivation: Heat inactivate enzyme at 70C for 20 minutes Storage: Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C. Unit Definition: 5 U of enzyme will digest 1 µg of genomic DNA in 25 µl reaction volume at 37C for ≥ 1 hr
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