Product Description
Direct-zol-96 RNA Product Supplied
with 200 ml x 2 TRI Reagent, 4 x 96 Preps
Zymo R2057
Storage Temperature: Room Temperature
The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).
Easy Handling: Bypass chloroform, phase separation and precipitation steps.
NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
Non-Biased: Complete RNA recovery without miRNA loss.
Compatibility: TRIzol, RNAzol, QIAzol, TriPure, TriSure and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent.
Equipment: Microplate centrifuge, vortex
Sample Inactivation: TRI Reagent inhibits RNase activity and inactivates viruses and other infectious agents.
Sample Source: Any sample stored and preserved in TRI Reagent, TRIzol or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size Range: Total RNA ≥ 17 nt
Yield: 10 µg RNA (binding capacity), ≥10 µl (elution volume)