Direct-zol RNA Microprep with
50 ml TRI Reagent, 50 Preps
Zymo R2061

Direct-zol RNA Microprep with
50 ml TRI Reagent, 50 Preps
Zymo R2061

Storage Temperature: Room Temperature

The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.

Easy Handling: Bypass chloroform, phase separation and precipitation steps. NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included). Non-Biased: Complete RNA recovery without miRNA loss.

Compatibility: TRIzol, RNAzol, QIAzol, TriPure, TriSure and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent. Equipment: Microcentrifuge, vortex Sample Inactivation: TRI Reagent inhibits RNase activity and inactivates viruses and other infectious agents. Sample Source: Any sample stored and preserved in TRI Reagent, TRIzol or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). Size Range: Total RNA ≥ 17 nt Yield: 10 µg RNA (binding capacity), ≥6 µl (elution volume)