Product Description
![Direct-zol-96 MagBead RNA,<br> 4 x 96 Preps<br> Zymo R2102 Direct-zol-96 MagBead RNA,<br> 4 x 96 Preps<br> Zymo R2102](https://s.turbifycdn.com/aah/yhst-12533177228474/direct-zol-96-magbead-rna-4-x-96-preps-zymo-r2102-11.gif)
Direct-zol-96 MagBead RNA,
4 x 96 Preps
Zymo R2102
Storage Temperature: Room Temperature
The Direct-zol-96 MagBead RNA provides a high-throughput, magnetic bead based purification of high-quality RNA directly from samples in TRI Reagent and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small and non-coding RNAs (17-200 nt) is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product.
The procedure is easy: simply add Direct-zol Binding Buffer and MagBinding Beads to a sample in TRI Reagent, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
*U.S. Patent No. 9,051,563 and other pending patents.
High-throughput, magnetic bead-based purification of high-quality (DNA-free) total RNA directly from TRIzol, TRI Reagent and all other acid-guanidinium-phenol based reagents (RNAzol, QIAzol, TriPure, TriSure, etc.)
Bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA
Sample Sources: Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol reagents.
RNA Purity: A260/A280 >1.8, A260/A230 >1.8. Complete removal of DNA is performed with DNase I digestion
RNA Recovery: The RNA binding capacity is ~10 µg/20 µl MagBinding Beads.
RNA Storage: RNA eluted with the DNase/RNase-Free Water (provided) can be stored at ≤-70°C. The addition of RNase inhibitors (optional) is highly recommended for prolonged storage.
Equipment Needed: Automated liquid handler with heating element, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually.
RNA Size: RNAs ≥17 nucleotides.
Sample Inactivation: TRI Reagent inhibits RNase activity and inactivates viruses and other infectious agents.