5-Fluoroorotic Acid, Powder,
1 Gram
Zymo F9001-1
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Product Description
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5-Fluoroorotic Acid, Powder,
1 Gram
Zymo F9001-1
Molecular Weight: 174
Chemical Formula: C5H3FN2O4• H2O
Storage Temperature: -20°C
Using 5-Fluoroorotic Acid (5-FOA) for the counter-selection of yeast is a common genetic screening method. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. Otherwise nontoxic to yeast, 5-FOA is converted to the toxic form (i.e., 5-flurouracil) in strains expressing the functional URA3 gene coding for orotine-5-monophosphate decarboxylase that is involved in the synthesis of uracil. Yeast strains that are phenotypically Ura+ become Ura- and 5-FOA(R) (resistant) after selection. The question of 5-FOA solubility is often raised by customers using ultra-pure (< 98%) 5-FOA powder because of its insolubility in water. Thus, we provide a 100X concentrated (100 mg/ml) 5-FOA solution in DMSO and synthetic 5-FOA monohydrate powder. Both have been tested and validated on the basis of counter selection activity.
Yeast Genetic Counter-Selection Agent: Commonly used for curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens.
Convenient: Available as a pure powder or ready-to-use solution in DMSO.
Ultra-Pure: Determined > 98% by thin-layer chromatography (TLC), melting point, and lot comparison.
Format: Synthetic 5-FOA monohydrate powder or 100X liquid (100 mg/ml in DMSO)
Molecular Weight: 174 g/mol
Product Storage: Store at -20°C
Purity: ≥ 98% (estimated) by TLC, melting point, and lot comparison
Solubility: 50 mg in 1 ml (1:1 1:1 NH4 OH:H2O) with gentle heating; >100 mg/ml in DMSO
Structure: C5H3FN2O4• H2O
Accessories
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![100X 5-Fluoroorotic Acid, Liquid, <br> 10 ml <br> Zymo F9003 100X 5-Fluoroorotic Acid, Liquid, <br> 10 ml <br> Zymo F9003](https://s.turbifycdn.com/aah/yhst-12533177228474/100x-5-fluoroorotic-acid-liquid-10-ml-zymo-f9003-12.gif) Regular price: $105.00 Sale price: $94.99 |