Product Description
ZymoBIOMICS RNA Mini Kit,
50 Preps
Zymo R2001
Storage Temperature: Room Temperature
The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.
Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
Ultra-Pure: Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
High Sensitivity: Increased detection limit of very low abundance organisms.
Equipment: Microcentrifuge, vortex, cell disrupter (recommended)
Purity: RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source: Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water.
Size Range: Total RNA ≥17 nt
Yield: 100 µg RNA (binding capacity), ≥50 µl (elution volume)