Product Description
TBS with Tween, 20X,
7.4 pH, Sterile Buffered, 1 Liter
RP-T59180-1000.0
Research or further manufacturing use only, not for food or drug use.
CAS Number: 7732-18-5; 77-86-1; 7647-14-5; 9005-64-5; 7547-01-0
Storage Temperature: Room Temperature
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is a commonly used buffer solution in the life science marketplace. TBS stands for Tris-Buffered Saline, and Tween refers to Tween 20, a nonionic detergent.
TBS with Tween is primarily used in various applications such as Western blotting, immunohistochemistry, and antibody-based assays. It serves as a buffer for sample dilution, antibody washing, and blocking steps during these experimental procedures.
The buffer solution is prepared at a 20X concentration, meaning it is twenty times more concentrated than the final desired concentration. This concentrated form allows for efficient storage and transportation.
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is specifically formulated to have a pH of 7.4, which is the physiological pH of many biological systems. This pH ensures compatibility with biological samples and minimizes interference with biological processes. The inclusion of Tween 20, a nonionic detergent, in the TBS buffer helps reduce nonspecific binding and improve the efficiency of antibody-antigen interactions.
It also aids in the washing and blocking steps by reducing background noise and enhancing signal detection. The buffer solution is sterile, ensuring aseptic conditions during experiments and minimizing the risk of contamination.
Appearance: Liquid
pH: 7.2 - 7.6