Product Description
Quick-DNA Fecal/Soil Microbe
DNA MidiPrep Kit, 25 Preps
Zymo ZD6110
The Quick-DNA Fecal/Soil Microbe Kits are designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.
Rapid methods for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes including those from humans, birds, rats, mice, cattle, etc.
Ultra-high density BashingBeads are fracture resistant and chemically inert.
Zymo-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.
Applicable For: All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume: ≥ 150 µl
Equipment: Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter
Processing Volume: ≤375 mg feces, ≤ 5 g soil, ≤ 500 mg fungal/bacterial cells (wet weight)
Purity: A260/A280 nm ≥1.8.
Sample Source: Feces or soil
Sample Storage: DNA stored at ≤ -20°C.
Size Range: Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Type: Total DNA
Yield: ≤ 125 µg total DNA