Product Description
Iron Stain Kit,
400 Slides
MER IRN1
The Iron Stain Kit is intended for use in the detection of ferric iron in tissues, blood smears, or bone marrow smears. Ferric iron is normally found in small amounts in bone marrow and the spleen. Abnormally large deposits may be seen in hemochromatosis and hemosiderosis. This product is based on the Prussian Blue reaction in which ionic iron reacts with acid ferrocyanide producing a blue color. Only ferric ions loosely bound to protein complexes will stain blue. Strongly bound ferric ions will not stain.
Kit Contents: 500mL Potassium Ferrocyanide Solution, 500mL Hydrochloric Acid Solution (2%), 125mL Nuclear Fast Red Solution
Expected Results: Iron (Bright Blue), Nuclei (Red), Background (Pink)
Suggested Controls: Spleen or Bone Marrow
Sideroblasts: These are nucleated erythrocytes containing at least one small blue granule. If the blue granules surround the nucleus, the cell is a ringed sideroblast.
Siderocytes: These are non-nucleated erythrocytes containing at least one blue granule.
Reticuloendothelial Iron: Usually seen as blue particles on the marrow smear or as blue particles in the cytoplasm or phagocytic cells.
Each component of the Iron Stain Kit may also be purchased separately